Monoclonal antibody generation using single B-cell screening for treating infectious diseases, in review (2023)

Development of a pan-tau multivalent nanobody that binds tau aggregation motifs and recognizes pathological tau aggregates, in review (2023)

Reduction of monoclonal antibody viscosity using interpretable machine learning, in review (2023)

Human antibody polyreactivity is governed primarily by the heavy chain complementarity-determining regions, in review (2023)

Facile generation of biepitopic antibodies with intrinsic agonism for activating receptors in the tumor necrosis factor superfamily, in review (2023).

Flow cytometric isolation of drug-like conformational antibodies specific for amyloid fibrils, in review (2023). [link]

Bispecific antibody shuttles targeting CD98hc mediate efficient and long-lived brain delivery of IgGs, Cell Chem Biol (2023). [link] [news story]

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates, Front Immunol,  (2023). [link]

Optimization of therapeutic antibodies for reduced self-association and non-specific binding using interpretable machine learning, Nat Biomed Eng (2023). [link] [news story

Simplifying complex antibody engineering using machine learning, Cell Systems (2023). [link]

Position-Specific Enrichment Ratio Matrix scores predict antibody variant properties from deep sequencing data, Bioinformatics (2023). [link]

Computational optimization of antibody humanness and stability by systematic energy-based ranking, Nat Biomed Eng (2023). [link]

Characterization of pairs of toxic and nontoxic misfolded protein oligomers elucidates the structural determinants of oligomer toxicity in protein misfolding diseases, Acc Chem Res (2023). [link]

Assessing developability early in the discovery process for novel biologics, mAbs (2023). [link]

Approaches to expand the conventional toolbox for discovery and selection of drug-like antibodies, mAbs (2023). [link]

Unlocking the potential of agonist antibodies for treating cancer using antibody engineering, Trends Mol Med (2023). [link]

Editorial overview: Nanobiotechnology, Curr Opin Biotech (2022). [link]

Reduction of therapeutic antibody self-association using yeast-display selections and machine learning, mAbs (2022). [link] [press release]

Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space, Nat Commun (2022). [link] [blog] [press release]

Mutational analysis of SARS-CoV-2 Variants of Concern reveals key tradeoffs between receptor affinity and antibody escape, PLOS Comput Biol (2022). [link]

Isolating anti-amyloid antibodies from yeast-displayed libraries, Methods Mol Biol (2022). [link]

Rapid and quantitative in-vitro evaluation of SARS-CoV-2 neutralizing antibodies and nanobodies, Anal Chem (2022). [link]

Antibodies with weakly basic isoelectric points minimize trade-offs between formulation and physiological colloidal properties, Mol Pharm (2022). [link]

Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis, STAR Protoc (2022). [link]

Improving antibody drug development using bionanotechnology, Curr Opin Biotech (2022). [link]

Agonist antibody discovery: experimental, computational and rational engineering approaches, Drug Discov Today (2022). [link]

A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain, PLoS One (2021). [link]

Discovery and characterization of high-affinity, potent SARS-CoV-2 neutralizing antibodies via single B cell screening, Sci Rep (2021). [link]

Systematic engineering of optimized autonomous heavy-chain variable domains, J Mol Biol (2021). [link]

Engineered multivalent nanobodies potently and broadly neutralize SARS-CoV-2 variants, Adv Ther (2021). [link]

Highly sensitive detection of antibody non-specific interactions using flow cytometry, mAbs (2021). [link]

Discovery-stage identification of drug-like antibodies using emerging experimental and computational methods, mAbs (2021). [link]

Ultra-dilute measurements of self-association for the identification of antibodies with favorable high concentration solution properties, Mol Pharm (2021). [link]

Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis, Cell Chem Biol (2021). [link][news story]

Rational affinity maturation of anti-amyloid antibodies with high conformational and sequence specificity, J Biol Chem (2021). [link]

Directed evolution of conformation-specific antibodies for sensitive detection of polypeptide aggregates in therapeutic drug formulations, Biotech Biogen (2021). [link]

Toward in silico CMC: An industrial collaborative approach to model‐based process development, Biotech Biogen (2020). [link]

Toward drug-like multispecific antibodies by design, Int J Mol Sci (2020). [link]

Physicochemical rules for identifying monoclonal antibodies with drug-like specificity, Mol Pharm (2020). [link]

Directed evolution methods for overcoming trade-offs between protein activity and stability, AIChE Journal (special issue honoring Frances Arnold's Nobel Prize) (2020). [link]

Unique impacts of methionine oxidation, tryptophan oxidation and asparagine deamidation on antibody stability and aggregation, J Pharm Sci (2020). [link]

An engineered, pH-toggle switch, human Fc domain for ultra-long circulation persistence , Nat Commun (2019). [link] 

Sensitive detection of glucagon aggregation using amyloid fibril-specific antibodies, Biotech Bioeng,  (2019). [link]

Nature-inspired design and evolution of anti-amyloid antibodies, J Biol Chem (2019). [link]

Deamidation compromises antibody colloidal stability and enhances aggregation in a pH-dependent manner, Mol Pharm (2019). [link]

Selecting and engineering monoclonal antibodies with drug-like specificity, Current Opin Biotech (2019). [link]

Net charge of the complementarity-determining regions is a key predictor of antibody specificity, Protein Eng Des Sel (2018). [link]

Understanding and overcoming trade-offs between antibody affinity, specificity, stability and solubility, Biochem Eng J (2018). [link]

Biophysical and sequence-based methods for identifying monovalent and bivalent antibodies with high colloidal stability, Mol Pharm (2018). [link]

Facile affinity maturation of antibody variable domains using natural diversity mutagenesis, Front Immunol (2017). [link]

Arginine mutations in antibody complementarity-determining regions display context-dependent affinity/specificity trade-offs, J Biol Chem (2017). [link]

Efficient affinity maturation of antibody variable domains requires co-selection of compensatory mutations to maintain thermodynamic stability, Sci Rep (2017). [link]

Glycan determinants of heparin-tau interaction, Biophys J (2017). [link]

Engineered autonomous human variable domains, Curr Pharm Des (2016). [link]

Facile preparation of stable antibody-gold conjugates and application to affinity-capture self-interaction nanoparticle spectroscopy, Bioconjug Chem (2016). [link]

Measurements of monoclonal antibody self-association are correlated with complex biophysical properties, Mol Pharm (2016). [link]

Intrahippocampal administration of a domain antibody that binds aggregated amyloid-β reverses cognitive deficits produced by diet-induced obesity, Biochim Biophys Acta (2016). [link]

Design and optimization of anti-amyloid domain antibodies specific for Aβ and IAPP, J Biol Chem (2016). [link]

Advances in antibody design, Ann Rev Biomed Eng (2015). [link] 

Co-evolution of affinity and stability of grafted amyloid-motif domain antibodies, Protein Eng Des Sel (2015). [link]

Discovery of highly soluble antibodies prior to purification using affinity-capture self-interaction nanoparticle spectroscopy, Protein Eng Des Sel (2015). [link]

Comparison of human and bovine insulin amyloidogenesis under uniform shear, J Phys Chem B (2015). [link]

An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies, mAbs (2015). [link]

High-throughput assay for measuring monoclonal antibody self-association and aggregation in serum, Bioconjug Chem (2015). [link] 

Improving monoclonal antibody selection and engineering using measurements of colloidal protein interactions, J Pharm Sci (2014). [link]

Plasmonic measurements of monoclonal antibody self-association using self-interaction nanoparticle spectroscopy, Biotech Bioeng (2014). [link]

Emerging methods for identifying monoclonal antibodies with low propensity to self-associate during the early discovery process, Expert Opin Drug Deliv (2014). [link]

Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold, Protein Eng Des Sel (2014). [link]

High-throughput screening for developability during early-stage antibody discovery using self-interaction nanoparticle spectroscopy, mAbs (2014). [link]

Mechanisms of transthyretin inhibition of Aβ aggregation in vitro, J Neurosci (2013). [link]

Modulation of curli assembly and pellicle biofilm formation by chemical and protein chaperones, Chem Biol (2013). [link] 

Toward aggregation-resistant antibodies by design, Trends Biotechnol (2013). [link]

Lifting the veil on amyloid drug design, eLife (2013). [link]

Rapid analysis of antibody self-association in complex mixtures using immunogold conjugates, Mol Pharm (2013). [link]

Rational design of potent domain antibody inhibitors of amyloid fibril assembly, P Natl Acad Sci U S A (2012). [link]

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions, Protein Eng Des Sel (2012). [link]

Conformational differences between two amyloid β oligomers of similar size and dissimilar toxicity, J Biol Chem (2012). [link]

Polyphenolic disaccharides endow proteins with unusual resistance to aggregation, Biotech Bioeng (2012). [link]

Engineering aggregation-resistant antibodies, Ann Rev Chem Biomol Eng (2012). [link]

Solution pH that minimizes self-association of three monoclonal antibodies is strongly dependent on ionic strength, Mol Pharm (2012). [link]

Structure-based design of conformation- and sequence-specific antibodies against amyloid β, P Natl Acad Sci U S A (2012). [link]

Removal versus fragmentation of amyloid-forming precursors via membrane filtration, Biotech Bioeng (2012). [link]

High-throughput analysis of concentration-dependent antibody self-association, Biophys J (2011). [link]

Site-specific structural analysis of a yeast prion strain with species-specific seeding activity, Prion (2011). [link]

Polyphenolic glycosides and aglycones utilize opposing pathways to selectively remodel and inactivate toxic oligomers of amyloid β, ChemBioChem (2011). [link]

Mutational analysis of domain antibodies reveals aggregation hotspots within and near the complementarity determining regions, Proteins (2011). [link]

Aromatic small molecules remodel toxic soluble oligomers of amyloid ß through three independent pathways, J Biol Chem (2011). [link]

Resveratrol selectively remodels soluble oligomers and fibrils of amyloid Aß into off-pathway conformers, J Biol Chem (2010). [link]

Unraveling molecular interactions and structures of self-perpetuating prions and amyloids, Nat Struct Mol Biol (2009). [link]

Biospecific immobilization of proteins for rapid analysis of weak protein interactions using self-interaction nanoparticle spectroscopy, Biotechnol Bioeng (2009). [link]

Self-interaction nanoparticle spectroscopy: A nanoparticle-based protein interaction assay, J Am Chem Soc (2008). [link]

Prion recognition elements govern nucleation, strain specificity and species barriers, Nature (2007). [link] *See accompanying commentaries in Nature (2007) [link]Nat Methods (2007) [link] & J Cell Biol (2007) [link].

Direct measurement of protein osmotic second virial cross coefficients by cross-interaction chromatography, Protein Sci (2004). [link]

Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients, Proteins (2003). [link]

Measurement of protein self-association as a guide to crystallization, Curr Opin Biotechnol (2003). [link]

Correlation of diafiltration sieving behavior of lysozyme-BSA mixtures with osmotic second virial cross coefficients, Biotechol Bioeng (2003). [link]

Rapid measurement of protein osmotic second virial coefficients by self-interaction chromatography, Biophys J (2002). [link]

Self-interaction chromatography: A novel screening method for rational protein crystallization, Acta Cryst D (2002). [link]

On-line spectroscopic characterization of sodium cyanide with nanostructured gold SERS substrates, Appl Spectrosc (2002). [link]

Structured metallic films for optical and spectroscopic applications via colloidal crystal templating, Adv Mater (2001). [link]

Assembly of gold nanostructured films templated by colloidal crystals and use in surface-enhanced Raman spectroscopy, J Am Chem Soc (2000). [link]

A class of porous metallic nanostructures, Nature (1999). [link]