Publications

An engineered, pH-toggle switch, human Fc domain for ultra-long circulation persistence , Nat Commun (2019). [link]

Unique impacts of methionine oxidation, tryptophan oxidation and asparagine deamidation on antibody stability and aggregation, J Pharm Sci (2019). [link]

Directed evolution methods for overcoming trade-offs between protein activity and stability, AIChE Journal (special issue honoring Frances Arnold's Nobel Prize) (2019). [link]

Sensitive detection of glucagon aggregation using amyloid fibril-specific antibodies, Biotech Bioeng, (2019). [link]

Nature-inspired design and evolution of anti-amyloid antibodies, J Biol Chem (2019). [link]

Net charge of the complementarity-determining regions is a key predictor of antibody specificity, Protein Eng Des Sel (2019). [link]

Deamidation compromises antibody colloidal stability and enhances aggregation in a pH-dependent manner, Mol Pharm (2019). [link]

Selecting and engineering monoclonal antibodies with drug-like specificity, Current Opin Biotech (2019). [link]

Understanding and overcoming trade-offs between antibody affinity, specificity, stability and solubility, Biochem Eng J (2018). [link]

Biophysical and sequence-based methods for identifying monovalent and bivalent antibodies with high colloidal stability, Mol Pharm (2018). [link]

Facile affinity maturation of antibody variable domains using natural diversity mutagenesis, Front Immunol (2017). [link]

Arginine mutations in antibody complementarity-determining regions display context-dependent affinity/specificity trade-offs, J Biol Chem (2017). [link]

Efficient affinity maturation of antibody variable domains requires co-selection of compensatory mutations to maintain thermodynamic stability, Sci Rep (2017). [link]

Glycan determinants of heparin-tau interaction, Biophys J (2017). [link]

Engineered autonomous human variable domains, Curr Pharm Des (2016). [link]

Facile preparation of stable antibody-gold conjugates and application to affinity-capture self-interaction nanoparticle spectroscopy, Bioconjug Chem (2016). [link]

Measurements of monoclonal antibody self-association are correlated with complex biophysical properties, Mol Pharm (2016). [link]

Intrahippocampal administration of a domain antibody that binds aggregated amyloid-β reverses cognitive deficits produced by diet-induced obesity, Biochim Biophys Acta (2016). [link]

Design and optimization of anti-amyloid domain antibodies specific for Aβ and IAPP, J Biol Chem (2016). [link]

Advances in antibody design, Ann Rev Biomed Eng (2015). [link]

Co-evolution of affinity and stability of grafted amyloid-motif domain antibodies, Protein Eng Des Sel (2015). [link]

Discovery of highly soluble antibodies prior to purification using affinity-capture self-interaction nanoparticle spectroscopy, Protein Eng Des Sel (2015). [link]

Comparison of human and bovine insulin amyloidogenesis under uniform shear, J Phys Chem B (2015). [link]

An alternative assay to hydrophobic interaction chromatography for high-throughput characterization of monoclonal antibodies, mAbs (2015). [link]

High-throughput assay for measuring monoclonal antibody self-association and aggregation in serum, Bioconjug Chem (2015). [link]

Improving monoclonal antibody selection and engineering using measurements of colloidal protein interactions, J Pharm Sci (2014). [link]

Plasmonic measurements of monoclonal antibody self-association using self-interaction nanoparticle spectroscopy, Biotech Bioeng (2014). [link]

Emerging methods for identifying monoclonal antibodies with low propensity to self-associate during the early discovery process, Expert Opin Drug Deliv (2014). [link]

Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold, Protein Eng Des Sel (2014). [link]

High-throughput screening for developability during early-stage antibody discovery using self-interaction nanoparticle spectroscopy, mAbs (2013). [link]

Mechanisms of transthyretin inhibition of Aβ aggregation in vitro, J Neurosci (2013). [link]

Modulation of curli assembly and pellicle biofilm formation by chemical and protein chaperones, Chem Biol (2013). [link]

Toward aggregation-resistant antibodies by design, Trends Biotechnol (2013). [link]

Lifting the veil on amyloid drug design, eLife (2013). [link]

Rapid analysis of antibody self-association in complex mixtures using immunogold conjugates, Mol Pharm (2013). [link]

Rational design of potent domain antibody inhibitors of amyloid fibril assembly, P Natl Acad Sci U S A (2012). [link]

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions, Protein Eng Des Sel (2012). [link]

Conformational differences between two amyloid β oligomers of similar size and dissimilar toxicity, J Biol Chem (2012). [link]

Polyphenolic disaccharides endow proteins with unusual resistance to aggregation, Biotech Bioeng (2012). [link]

Engineering aggregation-resistant antibodies, Ann Rev Chem Biomol Eng (2012). [link]

Solution pH that minimizes self-association of three monoclonal antibodies is strongly dependent on ionic strength, Mol Pharm (2012). [link]

Structure-based design of conformation- and sequence-specific antibodies against amyloid β, P Natl Acad Sci U S A (2012). [link]

Removal versus fragmentation of amyloid-forming precursors via membrane filtration, Biotech Bioeng (2012). [link]

High-throughput analysis of concentration-dependent antibody self-association, Biophys J (2011). [link]

Site-specific structural analysis of a yeast prion strain with species-specific seeding activity, Prion (2011). [link]

Polyphenolic glycosides and aglycones utilize opposing pathways to selectively remodel and inactivate toxic oligomers of amyloid β, ChemBioChem (2011). [link]

Mutational analysis of domain antibodies reveals aggregation hotspots within and near the complementarity determining regions, Proteins (2011). [link]

Aromatic small molecules remodel toxic soluble oligomers of amyloid ß through three independent pathways, J Biol Chem (2011). [link]

Resveratrol selectively remodels soluble oligomers and fibrils of amyloid Aß into off-pathway conformers, J Biol Chem (2010). [link]

Unraveling molecular interactions and structures of self-perpetuating prions and amyloids, Nat Struct Mol Biol (2009). [link]

Biospecific immobilization of proteins for rapid analysis of weak protein interactions using self-interaction nanoparticle spectroscopy, Biotechnol Bioeng (2009). [link]

Self-interaction nanoparticle spectroscopy: A nanoparticle-based protein interaction assay, J Am Chem Soc (2008). [link]

Prion recognition elements govern nucleation, strain specificity and species barriers, Nature (2007). [link] *See accompanying commentaries in Nature (2007) [link], Nat Methods (2007) [link] & J Cell Biol (2007) [link].

Direct measurement of protein osmotic second virial cross coefficients by cross-interaction chromatography, Protein Sci (2004). [link]

Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients, Proteins (2003). [link]

Measurement of protein self-association as a guide to crystallization, Curr Opin Biotechnol (2003). [link]

Correlation of diafiltration sieving behavior of lysozyme-BSA mixtures with osmotic second virial cross coefficients, Biotechol Bioeng (2003). [link]

Rapid measurement of protein osmotic second virial coefficients by self-interaction chromatography, Biophys J (2002). [link]

Self-interaction chromatography: A novel screening method for rational protein crystallization, Acta Cryst D (2002). [link]

On-line spectroscopic characterization of sodium cyanide with nanostructured gold SERS substrates, Appl Spectrosc (2002). [link]

Structured metallic films for optical and spectroscopic applications via colloidal crystal templating, Adv Mater (2001). [link]

Assembly of gold nanostructured films templated by colloidal crystals and use in surface-enhanced Raman spectroscopy, J Am Chem Soc (2000). [link]

A class of porous metallic nanostructures, Nature (1999). [link]